Medical ozone on hamstring injury in a professional athlete assessed by thermography: a clinical case report.

Injuries associated with the hamstring muscles in the running athlete are increasingly investigated due to the economic and functional consequences associated with them. Although hardly used in the treatment of sports injuries, medical ozone is effective and very well tolerated in the treatment of musculoskeletal pain, it was decided to add a series of medical ozone infiltrations to the treatment. The evolution of the case was recorded by medical thermography, in addition to measuring pain intensity (visual analog scale) and functional capacity (toe touch test). Pain intensity (visual analog scale) decreased from seven at baseline to two at the end of treatment (after two ozone infiltrations, one weekly). Mobility of the damaged area (toe touch test) improved from a distance of 8 cm at baseline to 0 cm at the end of treatment. Regarding medical thermography, after the first and second infiltration of ozone, the temperature rose to a significant increase in perfusion from baseline from 31.2 to 31.8 °C and from 31.2 to 32 °C, respectively. These results suggest the possible interest of medical ozone as an adjuvant treatment for the recovery of sports tendinopathies and encourage us to carry out further studies.

Efectos protectores de la Ozonoterapia en el daño renal y hepático en la Diabetes Mellitus Tipo 2 / Protective effects of Ozone therapy on Diabetes Mellitus type 2 renal and hepatic damage

La Diabetes Mellitus tipo 2 cursa con grandes complicaciones graves como lo son la insuficiencia renal crónica, la hepatopatía, la neuropatía diabética y la enfermedad cerebrovascular relacionadas con una disfunción metabólica que conlleva a un estado de inflamación crónica en estos pacientes. Con el objetivo de evaluar la influencia de la ozonoterapia rectal como terapia complementaria en pacientes con diabetes Mellitus Tipo 2, se realizó un ensayo observacional en 38 pacientes con Diabetes Mellitus tipo 2. Los pacientes fueron distribuidos aleatoriamente en dos grupos, el primer grupo recibió tratamiento convencional (Metformina, Glibenclamida y vitaminas del complejo B) y ozonoterapia rectal y al segundo (Convencional) se le prescribió solamente tratamiento convencional. Se realizaron determinaciones en sangre de glicemia, hemoglobina glicosilada, LDL-colesterol, VLDL-colesterol y triglicéridos, además se les midió marcadores de daño hepático, de daño renal y la escala DNA-4 de dolor neuropático, al inicio y al finalizar las 20 sesiones de ozonoterapia rectal. El grupo de pacientes que recibió ozonoterapia rectal más el tratamiento convencional mostrό disminución significativa (p 0,05) los valores de glicemia, hemoglobina glicosilada, VLDL-colesterol, triglicérido, LDL ­colesterol con respecto al grupo con tratamiento convencional. La ozonoterapia rectal, redujo significativa los marcadores de daño hepático y renal al igual que la escala de dolor DN4, con respecto al grupo que solamente recibió tratamiento convencional. La aplicación complementaria de Ozonoterapia rectal mostró un efecto protector sobre la función metabólica, hepática y renal en pacientes portadores de Diabetes Mellitus tipo 2, además mostró un cierto carácter analgésico mediante la reducción del dolor en dicha enfermedad.

Diabetes Mellitus Type 2 presents with major serious complications such as chronic kidney failure, liver disease, diabetic neuropathy, cerebrovascular disease created by metabolic dysfunction that leads to a state of chronic inflammation in these patients. With the objective of evaluating the influence of rectal ozone therapy as complementary therapy in patients with Type 2 diabetes Mellitus. 38 patients suffering Diabetes Mellitus Type 2 were selected and randomly divided into two groups. The first group (Ozone) received conventional treatment plus rectal ozone therapy and the second (Conventional) only received conventional treatment. Blood glucose, glycosylated hemoglobin, LDL-cholesterol, VLDL-cholesterol and triglycerides were measured in blood, markers of liver and kidney damage and the DNA-4 scale of neuropathic pain were measured at the beginning and at end of treatments with rectal ozone therapy. The group of patients who received rectal ozone therapy plus conventional treatment showed a significant decrease in glycemic, glycated hemoglobin, VLDL-cholesterol, triglyceride, LDL-cholesterol values compared to the group with conventional treatment. It reflected significant improvement in the liver and kidney damage markers as well as the levels of the DNA.-4 pain scale, compared to the group that only received conventional treatment. The complementary application of rectal ozone therapy showed a protective effect on metabolic, liver and kidney function in patients with Type 2 Diabetes Mellitus, and also showed a certain analgesic character by reducing pain in said disease.

Systemic Ozone Therapy by Rectal Insufflation for Immunoglobulin A Deficiency.

INTRODUCTION: IgA deficiency is a primary immunodeficiency predominantly due to an antibody defect, for which there is no replacement therapy. Treatment consists of prevention and treatment of infections and other associated conditions. Given the immunomodulatory and regulatory properties of the redox balance of ozone therapy in infectious and inflammatory conditions, evaluation of its effect on IgA deficiency is of interest. OBJECTIVE: Assess the benefits and possible adverse effects of ozone treatment in patients with IgA deficiency. METHODS: A monocentric randomized controlled phase 2 clinical trial (RPCEC 00000236) was carried out, after approval by the Institutional Ethics Committee of the Roberto Rodríguez Fernández Provincial General Teaching Hospital in Morón, Ciego de Ávila Province, Cuba. Included were 40 patients aged 5-50 years, distributed in 2 groups of 20, after agreeing to participate and signing informed consent. The experimental group received 2 cycles of ozone by rectal insufflation for 20 days (5 times a week for 4 weeks each cycle) with a 3-month interval between cycles, for a total of 40 doses, with age-adjusted dose ranges. The control group was treated with leukocyte transfer factor (Hebertrans), 1 U per m2 of body surface area subcutaneously, once weekly for 12 weeks. Frequency of appearance and severity of clinical symptoms and signs of associated diseases, serum immunoglobulin concentrations and balance of pro-oxidant and antioxidant biomarkers were recorded at treatment initiation and one month after treatment completion. Therapeutic response was defined as complete, partial, stable disease or progressive disease. Descriptive statistics and significance were calculated to compare groups and assess effect size. RESULTS: One month after treatment completion, 70% of patients in the experimental group experienced significant increases in IgG(p = 0.000) and IgM (p = 0.033). The experimental group also displayed decreased pro-oxidation biomarkers, glutathione modulation and increased antioxidant enzymes, with reduced oxidative stress; none of these occurred in the control group. Complete therapeutic response was achieved in 85% of patients in the experimental group and only 45% in the control group. Mild, transient adverse events were reported in both groups. CONCLUSIONS: Ozone therapy by rectal insufflation is a suitable therapeutic option for treating IgA deficiency because it produces antioxidant and immunomodulatory effects and is feasible, safe and minimally invasive. CONTRIBUTION OF THIS RESEARCH: This paper introduces in Cuba a new treatment a for IgA deficiency, with immunomodulatory and antioxidant effects offering substantial clinical benefits to patients with this immunodeficiency.

Efecto modulador de la ozonoterapia sobre la actividad del sistema inmune / Modulator effect of ozone therapy over immune system activity

El empleo de la medicina biológica ha permitido aplicar terapias sistémicas beneficiosas donde el cuerpo es tratado como un todo. La ozonoterapia es una técnica interesante en el campo de aplicación de la medicina biológica; es un proceder terapéutico, seguro, natural y factible, con resultados alentadores en el tratamiento de enfermedades inmunológicas, por el impacto social y económico que se genera tras su aplicación. Existen evidencias experimentales que aseveran esta afirmación. En este artículo se describen las propiedades inmunomoduladoras de la ozonoterapia, al caracterizar los efectos biológicos del ozono sobre las células del sistema inmune, mediadores solubles y otros tipos celulares(AU)

The employment of biological medicine has allowed the application of beneficial and systemic therapies where the body is treated as a whole. The ozone therapy is a modality of the biological medicine, it is a natural therapeutical procedure that has represented an extremely safe medical therapy, free from side effects, an effective and feasible method in the field of treatment of immunological diseases, with encouraging results for the social and economic impact that is generated after its application. There are experimental evidences that assert this statement. We hereby describe the immunomodulatory property of ozone therapy, the biological effects of the ozone on the cells of the immune system cells, other cellular types and different immune mediators(AU)

Efecto modulador de la ozonoterapia sobre la actividad del sistema inmune / Modulator effect of ozone therapy over immune system activity

El empleo de la medicina biológica ha permitido aplicar terapias sistémicas beneficiosas donde el cuerpo es tratado como un todo. La ozonoterapia es una técnica interesante en el campo de aplicación de la medicina biológica; es un proceder terapéutico, seguro, natural y factible, con resultados alentadores en el tratamiento de enfermedades inmunológicas, por el impacto social y económico que se genera tras su aplicación. Existen evidencias experimentales que aseveran esta afirmación. En este artículo se describen las propiedades inmunomoduladoras de la ozonoterapia, al caracterizar los efectos biológicos del ozono sobre las células del sistema inmune, mediadores solubles y otros tipos celulares

The employment of biological medicine has allowed the application of beneficial and systemic therapies where the body is treated as a whole. The ozone therapy is a modality of the biological medicine, it is a natural therapeutical procedure that has represented an extremely safe medical therapy, free from side effects, an effective and feasible method in the field of treatment of immunological diseases, with encouraging results for the social and economic impact that is generated after its application. There are experimental evidences that assert this statement. We hereby describe the immunomodulatory property of ozone therapy, the biological effects of the ozone on the cells of the immune system cells, other cellular types and different immune mediators

Ozone therapy as add-on treatment in fibromyalgia management by rectal insufflation: an open-label pilot study.

OBJECTIVES: The objectives of this study were to evaluate the effectiveness and tolerability of ozone therapy by rectal insufflation as add-on therapy in fibromyalgia management. DESIGN: Patients with fibromyalgia received 24 sessions of ozone therapy during a 12-week period. At each session, the administered dose of ozone was 8 mg (200 mL of gas, at a concentration of 40 µg/mL). Ozone sessions were given 5 days a week during the first 2 weeks, twice a week from weeks 3-6, and weekly from weeks 7-12. Fibromyalgia Impact Questionnaire (FIQ) was the main outcome measure, and was administered at baseline and at weeks 4, 8, and 12. Secondary outcome measures, administered at baseline and at endpoint, were the Pittsburgh Sleep Quality Index, the Beck Depression Inventory, the State and Trait Anxiety Inventory, and the SF-12, the abbreviated form of the Short Form Health Survey. Emergent adverse reactions to treatment were recorded. RESULTS: FIQ total scores decreased significantly during the study period, with the decrease being observed in the first 4 weeks of the study. Significant improvement was also seen both in depression scores and in the Physical Summary Score of the SF-12. Transient meteorism after ozone therapy sessions was the most frequently reported side-effect. CONCLUSIONS: At the dose and number of sessions used in this study, ozone therapy by rectal insufflation seems to be beneficial for physical symptoms and depression of fibromyalgia.

Efecto inmunomodulador de la ozonoterapia en niños con deficiencia en la inmunidad mediada por fagocitos / Immunomodulator effect of ozone therapy in children with deficiency in immunity mediated by phagocytes

Se realizó un estudio pre-experimental antes después, con la finalidad de evaluar el efecto inmunomodulador de la ozonoterapia en niños con inmunodeficiencia por defectos en la inmunidad mediada por fagocitos. El universo estuvo constituido por un total de 18 niños con deficiencias en la fagocitosis, asistidos en la consulta de Inmunología en el periodo desde septiembre de 2009 hasta junio de 2010, que cumplieron con los criterios de inclusión y exclusión. El ozono se aplicó en dosis escalonadas por insuflación rectal hasta una concentración de 40µg/mL, durante tres meses. Las variables fueron evaluadas 1 mes después del tratamiento. Se determinó si existen diferencias significativas entre las determinaciones antes y después de la ozonoterapia, mediante la prueba T de comparación de medias para muestras dependientes, con un nivel de significación α=0.05. Hubo aumentos significativos del conteo absoluto de neutrófilos en el 100 por ciento de los casos y en el 94 por ciento mejoró significativamente la función fagocítica leucocitaria después de recibir la ozonoterapia. El estado clínico, cualitativamente evaluado, fue satisfactorio en los niños tratados con Ozono y no se reportaron reacciones adversas durante el tratamiento. Se sugiere este proceder terapéutico como alternativa terapéutica inmunoestimulante en la deficiencia fagocítica(AU)

A pre-experimental study was conducted before then, in order to assess the effect of immunomodulator of ozone therapy in children with immunodeficiency defects of phagocyte-mediated immunity. The universe was made up by a total of 18 children with deficiencies in phagocytosis, assisted in the Immunology consultation from September 2009 until June 2010 that met the inclusion and exclusion criteria. Ozone was applied in doses by rectal insufflation stepped up a 40µg/mL concentration, for three months. The variables were assessed 1 month after treatment. It was determined if there are significant differences between determinations before and after of ozone therapy, through T of means comparison test for dependent samples, with a significance level α = 0. 05. There were significant increases in the absolute neutrophil count in 100 percent of cases and the Leukocyte Phagocytic function significantly improved in a 94 percent after receiving ozone therapy. The clinical state, qualitatively evaluated, was satisfactory in children treated with ozone and no adverse reactions were reported during treatment. It is suggested this therapeutic approach as immunostimulant therapeutic alternative in the phagocytic deficiency(AU)

Efecto inmunomodulador de la ozonoterapia en niños con deficiencia en la inmunidad mediada por fagocitos / Immunomodulator effect of ozone therapy in children with deficiency in immunity mediated by phagocytes

Se realizó un estudio pre-experimental antes después, con la finalidad de evaluar el efecto inmunomodulador de la ozonoterapia en niños con inmunodeficiencia por defectos en la inmunidad mediada por fagocitos. El universo estuvo constituido por un total de 18 niños con deficiencias en la fagocitosis, asistidos en la consulta de Inmunología en el periodo desde septiembre de 2009 hasta junio de 2010, que cumplieron con los criterios de inclusión y exclusión. El ozono se aplicó en dosis escalonadas por insuflación rectal hasta una concentración de 40µg/mL, durante tres meses. Las variables fueron evaluadas 1 mes después del tratamiento. Se determinó si existen diferencias significativas entre las determinaciones antes y después de la ozonoterapia, mediante la prueba T de comparación de medias para muestras dependientes, con un nivel de significación α=0.05. Hubo aumentos significativos del conteo absoluto de neutrófilos en el 100 por ciento de los casos y en el 94 por ciento mejoró significativamente la función fagocítica leucocitaria después de recibir la ozonoterapia. El estado clínico, cualitativamente evaluado, fue satisfactorio en los niños tratados con Ozono y no se reportaron reacciones adversas durante el tratamiento. Se sugiere este proceder terapéutico como alternativa terapéutica inmunoestimulante en la deficiencia fagocítica.

A pre-experimental study was conducted before then, in order to assess the effect of immunomodulator of ozone therapy in children with immunodeficiency defects of phagocyte-mediated immunity. The universe was made up by a total of 18 children with deficiencies in phagocytosis, assisted in the Immunology consultation from September 2009 until June 2010 that met the inclusion and exclusion criteria. Ozone was applied in doses by rectal insufflation stepped up a 40µg/mL concentration, for three months. The variables were assessed 1 month after treatment. It was determined if there are significant differences between determinations before and after of ozone therapy, through T of means comparison test for dependent samples, with a significance level α = 0. 05. There were significant increases in the absolute neutrophil count in 100 percent of cases and the Leukocyte Phagocytic function significantly improved in a 94 percent after receiving ozone therapy. The clinical state, qualitatively evaluated, was satisfactory in children treated with ozone and no adverse reactions were reported during treatment. It is suggested this therapeutic approach as immunostimulant therapeutic alternative in the phagocytic deficiency.

Ozone oxidative post-conditioning reduces oxidative protein damage in patients with disc hernia.

INTRODUCTION/OBJECTIVES: Although inflammation in disc hernia (DH) has been recognized and it is a well-known process mediated by loss of the cellular redox balance, only a few studies about the impact of chronic oxidative stress on this neurological disorder have been made. Ozone therapy has been widely used with clinical efficacy in DH. This work aimed at characterizing the systemic redox status of patients with low back pain and neck pain as well as studying if ozone oxidative post-conditioning modified the pathological oxidative stress and protected against oxidative protein damage and if there is any relationship between oxidative changes and pain in both DH. METHODS: Redox status of 33 patients with diagnosis of DH by computerized axial tomography, nuclear magnetic resonance, and clinical evaluations was studied. Ozone was administered by paravertebral way. After ozone treatment, plasmatic levels of antioxidant/pro-oxidant markers, pain, and life quality disability parameters were evaluated. RESULTS: One hundred percent of patients showed a severe oxidative stress. Major changes in superoxide dismutase activity, total hydroperoxides, advanced oxidation protein products, fructolysine content, and malondialdehyde were observed. After ozone oxidative post-conditioning, there was a re-establishment of patients' cellular redox balance as well as a decrease in pain in both DH. A relationship between indicators of oxidative protein damage and pain was demonstrated. CONCLUSIONS: Ozone therapy protected against oxidation of proteins and reduced the pain. Relationship between markers of oxidative protein damage, disability parameters, and pain suggests the role of oxidative stress in the pathological processes involved in DH.

Ozonoterapia y electroestimulación en retinosis pigmentaria / Ozone therapy and electrical stimulation in pigmentary retinitis

OBJETIVO: Profundizar en los efectos de la ozonoterapia y electroestimulación sobre la función visual de los pacientes que padecen retinosis pigmentaria. MÉTODOS: Se estudiaron 186 pacientes portadores de retinosis pigmentaria. Fueron agrupados atendiendo a los estadios clínicos de la clasificación cubana (4 grupos). Se aplicó ozonoterapia y electroestimulación, una sesión diaria de cada una durante 14 días. Se realizó un examen oftalmológico antes y después del tratamiento, para la agudeza visual se empleó cartilla de Snellen y para el campo visual cinético tipo Goldman. Los datos estadísticos se analizaron y compararon usando la prueba t de Student. Se consideró p < 0,05 como significación estadística. RESULTADOS: En los 57 pacientes agrupados en el estadio 1, la agudeza visual con corrección se mantuvo igual. Se observaron modificaciones significativas del campo visual en 16 pacientes. En los 66 pacientes agrupados en el estadio 2, la agudeza visual con corrección se mantuvo igual en 48 pacientes con modificaciones significativas del campo visual en 29 pacientes. En el estadio 3 se agruparon 49 pacientes donde la agudeza visual con corrección se mantuvo igual en 38 pacientes, observando modificaciones muy significativas del campo visual en 27 pacientes. CONCLUSIÓN: En la estrategia multiterapéutica cubana para el tratamiento de la retinosis pigmentaria, las aplicaciones cíclicas anuales de ozonoterapia y electroestimulación son imprescindibles para mantener un control de la enfermedad(AU)

OBJECTIVE: To analyze in depth the effects of ozone therapy and electrical stimulation on the visual function of patients suffering pigmentary retinitis. METHODS: One hundred and eighty six patients carrying pigmentary retinitis were studied. They were grouped according to the clinical staging of the Cuban classification (4 groups). Ozone therapy and electrical stimulations were applied daily for 14 days. An ophthalmologic test before and after the treatment was performed; Snellen´s charter for visual acuity and Goldman-type kinetic visual field. Statistical data were analyzed and compared using Student´s t test. The statistical significance was set at p<0,05. RESULTS: The corrected visual acuity remained the same in the 57 patients grouped in staging 1. Significant changes were observed in the visual field of 16 patients. Regarding the 66 patients in staging 2, corrected visual acuity remained the same in 48 whereas 29 patients experienced significant changes in their visual field. Forty nine patients were included in the staging 3 where corrected visual acuity kept the same value in 38 patients, but very significant changes in the visual field of 27 patients were observed. CONCLUSION: In the Cuban multitherapeutical strategy for the treatment of pigmentary retinitis, the annual cyclic use of ozone therapy and electrical stimulation is essential to keep control over the disease(AU)

Ozonoterapia y electroestimulación en retinosis pigmentaria / Ozone therapy and electrical stimulation in pigmentary retinitis

OBJETIVO: Profundizar en los efectos de la ozonoterapia y electroestimulación sobre la función visual de los pacientes que padecen retinosis pigmentaria. MÉTODOS: Se estudiaron 186 pacientes portadores de retinosis pigmentaria. Fueron agrupados atendiendo a los estadios clínicos de la clasificación cubana (4 grupos). Se aplicó ozonoterapia y electroestimulación, una sesión diaria de cada una durante 14 días. Se realizó un examen oftalmológico antes y después del tratamiento, para la agudeza visual se empleó cartilla de Snellen y para el campo visual cinético tipo Goldman. Los datos estadísticos se analizaron y compararon usando la prueba t de Student. Se consideró p < 0,05 como significación estadística. RESULTADOS: En los 57 pacientes agrupados en el estadio 1, la agudeza visual con corrección se mantuvo igual. Se observaron modificaciones significativas del campo visual en 16 pacientes. En los 66 pacientes agrupados en el estadio 2, la agudeza visual con corrección se mantuvo igual en 48 pacientes con modificaciones significativas del campo visual en 29 pacientes. En el estadio 3 se agruparon 49 pacientes donde la agudeza visual con corrección se mantuvo igual en 38 pacientes, observando modificaciones muy significativas del campo visual en 27 pacientes. CONCLUSIÓN: En la estrategia multiterapéutica cubana para el tratamiento de la retinosis pigmentaria, las aplicaciones cíclicas anuales de ozonoterapia y electroestimulación son imprescindibles para mantener un control de la enfermedad

OBJECTIVE: To analyze in depth the effects of ozone therapy and electrical stimulation on the visual function of patients suffering pigmentary retinitis. METHODS: One hundred and eighty six patients carrying pigmentary retinitis were studied. They were grouped according to the clinical staging of the Cuban classification (4 groups). Ozone therapy and electrical stimulations were applied daily for 14 days. An ophthalmologic test before and after the treatment was performed; Snellen´s charter for visual acuity and Goldman-type kinetic visual field. Statistical data were analyzed and compared using Student´s t test. The statistical significance was set at p<0,05. RESULTS: The corrected visual acuity remained the same in the 57 patients grouped in staging 1. Significant changes were observed in the visual field of 16 patients. Regarding the 66 patients in staging 2, corrected visual acuity remained the same in 48 whereas 29 patients experienced significant changes in their visual field. Forty nine patients were included in the staging 3 where corrected visual acuity kept the same value in 38 patients, but very significant changes in the visual field of 27 patients were observed. CONCLUSION: In the Cuban multitherapeutical strategy for the treatment of pigmentary retinitis, the annual cyclic use of ozone therapy and electrical stimulation is essential to keep control over the disease

Antioxidant mechanism is involved in the gastroprotective effects of ozonized sunflower oil in ethanol-induced ulcers in rats.

This research was performed in order to determine the potential protective effects of ozonized sunflower oil (OSO) in the injury of rat gastric mucosa induced by absolute ethanol and as well as to elucidate the role of reactive oxygen species (ROS), lipid peroxidation, and some important constituents of antioxidant defense such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in these effects. OSO was administered to rats intragastrically by a cannula and it was applied during four days to animals. The doses of OSO administered daily to each group of rats were 4, 12, and 24 mg/kg, respectively, and one hour after the last treatment, absolute ethanol (1 mL/200 mg body weight) was administered. Our results showed that gastric ulcer index was significantly reduced in rats pretreated with OSO as compared with ethanol-treated controls. However, in rats pretreated with OSO, no significant reduction of TBARS content in gastric mucosa was found as compared to those rats treated with ethanol alone. In contrast, SOD and GSH-Px activities were significantly increased in gastric mucosa of OSO-pretreated rats with respect to those treated with ethanol alone. In summary, our results demonstrate that OSO pretreatment exerts protective effects in ethanol-induced gastric ulcers in rats. Furthermore, these results provide evidence that these protective effects of OSO are mediated at least partially by stimulation of some important antioxidant enzymes such as SOD and GSH-Px, which are scavengers of ROS and therefore prevent gastric injury induced by them.

Antioxidant Mechanism is Involved in the Gastroprotective Effects of Ozonized Sunflower Oil in Ethanol-Induced Ulcers in Rats

This research was performed in order to determine the potential protective effects of ozonized sunflower oil (OSO) in the injury of rat gastric mucosa induced by absolute ethanol and as well as to elucidate the role of reactive oxygen species (ROS), lipid peroxidation, and some important constituents of antioxidant defense such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in these effects. OSO was administered to rats intragastrically by a cannula and it was applied during four days to animals. The doses of OSO administered daily to each group of rats were 4, 12, and 24 mg/kg, respectively, and one hour after the last treatment, absolute ethanol (1 mL/200 mg body weight) was administered. Our results showed that gastric ulcer index was significantly reduced in rats pretreated with OSO as compared with ethanol-treated controls. However, in rats pretreated with OSO, no significant reduction of TBARS content in gastric mucosa was found as compared to those rats treated with ethanol alone. In contrast, SOD and GSH-Px activities were significantly increased in gastric mucosa of OSO-pretreated rats with respect to those treated with ethanol alone. In summary, our results demonstrate that OSO pretreatment exerts protective effects in ethanol-induced gastric ulcers in rats. Furthermore, these results provide evidence that these protective effects of OSO are mediated at least partially by stimulation of some important antioxidant enzymes such as SOD and GSH-Px, which are scavengers of ROS and therefore prevent gastric injury induced by them(AU)

Estrés oxidativo en un modelo de ratón quemado tratado con Aloe b, ozono y factor de crecimiento epidérmico / Oxidative stress in a model of burnt mouse treated with Aloe b, ozone and epidermal growth factor

La enfermedad por quemadura estimula la respuesta inflamatoria sistémica y el daño múltiple de órganos, su expresión morfológica. Con el propósito de identificar el comportamiento del estrés oxidativo mediante el malondialdehído, la superóxido dismutasa y la catalasa y su modificación con el empleo del tratamiento sistémico con Aloe b, ozono o factor de crecimiento epidérmico, se elaboró un modelo de quemadura seca de un área de 11 por ciento, en ratones hembras, balb/c de 20 ± 2 g (n= 24). Se aplicó eutanasia en diferentes tiempos, se estudiaron los órganos con evaluación cualitativa en grados de intensidad y las variables bioquímicas se obtuvieron del riñón y del hígado. La prueba G se empleó para conocer la dependencia entre las variables y la prueba t se usó al comparar porcentajes. El análisis de varianza bifactorial y la prueba de rango múltiple de Duncan se empleó en las variables del estrés oxidativo estudiadas. Se siguieron las normas éticas en el trabajo con los animales. Existió dependencia entre los diferentes grupos y la vitalidad (G= 268,83***) superior en los grupos tratados. Los grupos no tratados tuvieron mayor intensidad del daño múltiple de órganos y peor respuesta del estrés oxidativo. Los grupos con mejor evolución fueron el tratado con ozono y el tratado con factor de crecimiento epidérmico. El tratamiento inmediatamente después de la quemadura con ozono y factor de crecimiento epidérmico resultó favorable para la evolución y supervivencia de los animales(AU)

Estrés oxidativo en un modelo de ratón quemado tratado con Aloe b, ozono y factor de crecimiento epidérmico / Oxidative stress in a model of burnt mouse treated with Aloe b, ozone and epidermal growth factor

La enfermedad por quemadura estimula la respuesta inflamatoria sistémica y el daño múltiple de órganos, su expresión morfológica. Con el propósito de identificar el comportamiento del estrés oxidativo mediante el malondialdehído, la superóxido dismutasa y la catalasa y su modificación con el empleo del tratamiento sistémico con Aloe b, ozono o factor de crecimiento epidérmico, se elaboró un modelo de quemadura seca de un área de 11 por ciento, en ratones hembras, balb/c de 20 ± 2 g (n= 24). Se aplicó eutanasia en diferentes tiempos, se estudiaron los órganos con evaluación cualitativa en grados de intensidad y las variables bioquímicas se obtuvieron del riñón y del hígado. La prueba G se empleó para conocer la dependencia entre las variables y la prueba t se usó al comparar porcentajes. El análisis de varianza bifactorial y la prueba de rango múltiple de Duncan se empleó en las variables del estrés oxidativo estudiadas. Se siguieron las normas éticas en el trabajo con los animales. Existió dependencia entre los diferentes grupos y la vitalidad (G= 268,83***) superior en los grupos tratados. Los grupos no tratados tuvieron mayor intensidad del daño múltiple de órganos y peor respuesta del estrés oxidativo. Los grupos con mejor evolución fueron el tratado con ozono y el tratado con factor de crecimiento epidérmico. El tratamiento inmediatamente después de la quemadura con ozono y factor de crecimiento epidérmico resultó favorable para la evolución y supervivencia de los animales

Ozone therapy effects on biomarkers and lung function in asthma.

BACKGROUND: The relationship and behavior of serum immunoglobulin E (IgE) level, peripheral blood mononuclear cell (PBMC) human leukocyte antigen DR (HLA-DR) expression and erythrocyte glutathione antioxidant pathway in asthma patients treated with systemic ozone therapy have not been studied before. METHODS: Asthma patients were treated about 1 year with three cycles (5 or 6 months each) with three different ozone therapy protocols. Ozone major autohemotherapy (MAHT) was applied at doses of 4 and 8 mg, 15 sessions each cycle; and ozone rectal insufflations (RI) at a dose of 10 mg, 20 sessions each cycle. Serum IgE, HLA-DR expression in PBMC and biomarkers for antioxidant pathway were measured before and at the end of each cycle. Lung function and symptoms test were recorded at the beginning and after the third cycle. RESULTS: IgE and HLA-DR decreased with the three types of treatments, while increments in reduced glutathione, glutathione peroxidase, glutation reductase and glutathione S-transferase were achieved with all treatments. Lung function and symptoms test were markedly improved. However, in all parameters the best response was obtained in the order: MAHT at 8 mg better than MAHT at 4 mg better than RI at 10 mg. Before ozone treatment, glutathione antioxidant parameters were under the normal reference values, suggesting the occurrence of oxidative stress associated with atopic asthma. CONCLUSIONS: This study demonstrates the effectiveness of ozone therapy in reducing IgE and inflammatory mediators along with the induction of antioxidant elements. The study raises the role of systemic ozone therapy in atopic asthma by means of its immunomodulatory and oxidative stress regulation properties.

Ozonoterapia en SIDA / Ozone therapy in AIDS

Se estudió un paciente SIDA IIIc con fatiga, síndrome de mala absorción intestinal, polineuropatía, anemia, con glucosa, creatinina y tiempo de protombina elevados, conteo CD4 en 37 células carga viral en 2 400 000/mL, el cual venía recibiendo un cóctel de triple terapia retroviral, anemia de 10 g/L. Se le administró un ciclo de ozono por vía rectal, a dosis de 5 mg, junto con interferón, factor de transferencia y eritropoyetina recombinante. Al término del tratamiento se le realizó una evaluación final donde se encontró que desaparecieron la fatiga, las diarreas, la deshidratación y la anemia, aumentando 8 kg de peso y se normalizaron los valores de glucosa, creatinina y tiempo de protombina. Después de 3 meses de finalizado el tratamiento con ozono, continuando con la administración ininterrumpida de interferones y una tetraterapia antirretroviral, el conteo CD4 aumentó a 546 con 30 por ciento y la carga viral disminuyó a 1 700/mL. Se valoró el efecto del ozono como inmunomodulador y antimicrobiano potente en pacientes VIH/SIDA(AU)

Ozonoterapia en SIDA / Ozone therapy in AIDS

Se estudió un paciente SIDA IIIc con fatiga, síndrome de mala absorción intestinal, polineuropatía, anemia, con glucosa, creatinina y tiempo de protombina elevados, conteo CD4 en 37 células carga viral en 2 400 000/mL, el cual venía recibiendo un cóctel de triple terapia retroviral, anemia de 10 g/L. Se le administró un ciclo de ozono por vía rectal, a dosis de 5 mg, junto con interferón, factor de transferencia y eritropoyetina recombinante. Al término del tratamiento se le realizó una evaluación final donde se encontró que desaparecieron la fatiga, las diarreas, la deshidratación y la anemia, aumentando 8 kg de peso y se normalizaron los valores de glucosa, creatinina y tiempo de protombina. Después de 3 meses de finalizado el tratamiento con ozono, continuando con la administración ininterrumpida de interferones y una tetraterapia antirretroviral, el conteo CD4 aumentó a 546 con 30 por ciento y la carga viral disminuyó a 1 700/mL. Se valoró el efecto del ozono como inmunomodulador y antimicrobiano potente en pacientes VIH/SIDA

Ozonoterapia y laserpuntura en el tratamiento de la sordera súbita / Ozone therapy and the laser puncture in the treatment of sudden deafness

Uno de los cuadros clínicos de instalación más impresionante que obligan al especialista en Otorrinolaringología a actuar bajo criterios de protocolo clínico y de tratamiento, es la aparición de una hipoacusia neurosensorial de instalación rápidamente progresiva. Causas vasculares, virales e inmunológicas, por sí solas o de manera interactiva, han sido señaladas como responsables de los trastornos que se originan, los cuales confluyen en la pérdida de la audición. Por todas estas razones, es objetivo evaluar los resultados obtenidos tras aplicar la combinación de dos métodos de tratamiento, los cuales, de acuerdo con la información de que se dispone, pueden ser efectivos en la atención de esta patología. Resultado significativo del protocolo de tratamiento fue una tasa de recuperación del 88 por ciento de los pacientes tratados(AU)

Ozonoterapia y laserpuntura en el tratamiento de la sordera súbita / Ozone therapy and the laser puncture in the treatment of sudden deafness

Uno de los cuadros clínicos de instalación más impresionante que obligan al especialista en Otorrinolaringología a actuar bajo criterios de protocolo clínico y de tratamiento, es la aparición de una hipoacusia neurosensorial de instalación rápidamente progresiva. Causas vasculares, virales e inmunológicas, por sí solas o de manera interactiva, han sido señaladas como responsables de los trastornos que se originan, los cuales confluyen en la pérdida de la audición. Por todas estas razones, nos dimos a la tarea de evaluar los resultados obtenidos tras aplicar la combinación de dos métodos de tratamiento, los cuales, de acuerdo con la información de que se dispone, pueden ser efectivos en la atención de esta patología. Resultado significativo del protocolo de tratamiento fue una tasa de recuperación del 88 por ciento de los pacientes tratados(AU)

One of the most impressive installation clinical pictures that obliges the Otorhinolaryngology specialist to act under the criteria of clinical protocol and treatment is the appearance of a rapidly progressive installation neurosensorial hypoacusia. Vascular, viral and immunological causes by themselves, or in an interactive manner, have been considered responsible for the disorders that are originated and that lead to a clinical picture of auditive loss. For all these reasons, we decided to evaluate the results obtained after applying the combination of two treatment methods, which, according to the available information, may be effective in the attention of this pathology. A significant result of the treatment protocol was the recovery rate of 88 percent of the treated patients(AU)